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I have a previously stable tank that has been up and running for about five years. I've not added any new fish for at least a year. Now my fish are dying off a few a day. Two two days ago, two yesterday, and five today.

I am aware that my tank is overstocked. I didn't realize it when I stocked it all those years ago, but it seemed to be fine until just now.

1. Size of tank?
49 Gal

2. Water parameters
a. Ammonia? 0
b. Nitrite? 0
c. Nitrate? 20
d. pH, KH and GH? 7.5, unknown, 120
e. Test kit? API

3. Temperature?
78 F

4. FW (fresh water) or BW (brackish)?
FW

5. How long the aquarium has been set up?
5+ years

6. What fish do you have? How many are in your tank?

2 Otocinclus Catfish
1 Twig catfish
1 powder blue dwarf gourami
5 assorted cory cats
5 zebra danios
4 glass catfish
5 whiteclouds
10 fancy guppies
11 assorted platies
7 neon tetras
5 black neon tetras
5 mollies
1 female betta
1 african dwarf frog
undetermined number of ghost shrimp

Dead Fish
2 guppies
my betta
2 mollies
1 danio
1 whitecloud
my ADF
1 black neon

7. Were the fish placed under quarantine period (minus the first batch from the point wherein the tank is ready to accommodate the inhabitants)?
No, but it was so long ago, I feel it is irrelevant.

8. a. Any live plants? Fake plants?
all live plants. no fake ones.

b. Sand, gravel, barebottom?
Gravel

c. Rocks, woods, fancy decors? Any hollow decors?
Real wood, rocks, and some hollow decor.

9. a. Filtration?
100 gallon cannister

10. a. Lighting schedule? What lights are used?
plant lighting, 8-10 hours a day
b. Any sunlight exposure? How long? Very small, if any.

11. a. Water change schedule? weekly
b. Volume of water changed? Around 10 Gallons
c. Well water, tap water, RO water? Tap Water
d. Water conditioner used? stress coat
e. Frequency of gravel/sand (if any) vacuumed? not vaccumed due to plants rooted in gravel

12. Foods? dried flake and spirulina wafers
How often are they fed? every other day

13. a. Any abnormal signs/symptoms?
nothing!

b. Appearance of poop? normal
c. Appearance of gills? Normal

14. a. Have you treated your fish ahead of diagnosis? No
b. What meds were used? n/a
 
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