I recommend adding it to the top so the benefical part can make immediate contact with the tank itself. It will quickly seed the rest. get LR from several places if possible as the rock will contain different organisms. the more varied the better.
This is my first fish tank that i set up, its a 55 gallon tank with a topfin power filter and a biological emperor power filter and 200 watt heater, i added a mix of live sand and unlive sand so far to it.
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