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1 every 4 sec is all u need if the thing is on all day- dont forget this will affect your pH!!! i would advise you remove it at night or you might experience pH crash. I wouldnt advise you use an airstone with CO2, its like peeing in the wind- it will displace the CO2 from the water.
 

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your amount of co2 in the tank will be small, there is already oxygen in the water- what is the chemical make up of water?

If you break the surface of the water or use an airstone you will displace any co2 you inject, so remove the airstone.

Only does small amounts of co2 to begin with - once you have a tank of plants your plants will produce the o2 for your fish.

You wont see any amano tanks with airstones in them, it all depends on the system and the type of tank you are trying to produce.
 
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