My biggest mistake had nothing to do with what I did, per se, but what I accidentally allowed to happen.
I worked at a pet store that sold fish- both salt water and fresh. During the 'Nemo' craze, a woman came in, bought a Beta bowl and asked me for a 'Percula Clownfish'. I did the regular, and asked the woman if she realized it was salt water, and she said "Oh yeah, I know". So I assumed she planned on putting it in a salt water tank.
about an hour later she called because the fish had gotten very ill, and it was because she put it into the beta bowl despite what I had told her.
I know I took the fish back, but I have no idea what else came of it, I don't remember. Still, this was definitely my biggest mistake.