01-31-2011, 01:42 PM
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A lot of that would depend on how good of a diffuser you have....
Without a diffuser at all, even 32 bubbles will be negligable.
With a diffuser, I'd start at 7 bubbles per minute, and maybe work up to 12-18 eventually (and raise it regularly by 2 bubbles a day or so. If you have an algae outbreak or if the fish gasp at the surface by the end of the day, lower it down and keep it there.) Another way to see if you have enough CO2, is to monitor the Ph drop. (Don't let it drop by more than .3 or so. Test the ph at the same time every day since ph fluctuates on its own.)
The problem with DIY CO2 is that it will vary. Having several bottles will help keep it stable.. Perhaps 3 bottles...
Start 1 on today, label it A.
In 2 days, start a second, and label it B.
In 2 more days, make another, bottle C.
2 days later, pour bottle A out and restart it.
You could use more bottles of course, but that should stabilise it.
Running the CO2 into a powerhead may or may not diffuse it properly... No way to tell.
Why you worrying about CO2?