06-05-2013, 07:41 AM
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Might consider that plant's are able to transport oxygen to their root's ,so lot's of plant's ,,,would negate any concern's over anaerobic (lack of oxygen) condition's for ME.
Will assume with the substrate mentioned,,that plant's are going in?
Many pond's, lakes,I have fished, have sand,mud,bottom's that nobody pokes,stir's,and yet the fishes thrive here year after year.
Me think's anything over five inches might encourage anerobic condition's but would be of small concern in heavily planted tank. IMHO