NitrItes are still zero four full days after dosing my tank with Prime. If Prime's ability to bind up excess ammonia and nitrite does wear-off, I don't see any indication that residual concentrations of those compounds are in the tank. Maybe I'm cycled!
I did no water changes during this period, and I have made no additions or subtractions to the tank.
Day Minus 1: 5.0 ppm or higher
Day 0: 5.0 ppm (administered Prime)
Day 1: <0.25 ppm
Day 2: 0 ppm
Day 3: 0 ppm
Day 4: 0 ppm
Ammonia has been under 0.25 ppm the whole time. BTW... I think it's zero ppm, but my wife believes she still sees a little green hue in the test results. It is definitely lighter than the reference color for 0.25 ppm on the API kit.
NitrIte reading is unambiguous... zero.
NitrAte is between 20 ppm and 40 ppm.