11-06-2011, 10:31 AM
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Since your 20g is a new tank setup, it would be some time with your stock before you would see an ammonia spike. Short of an API test kit, perhaps you can get a Seachem Ammonia Alert - they are inexpensive and pretty effective. In the event of an ammonia spike, be prepared to do a water change.
Just curious...did you use some gravel or filter material from the 5g tank to "bio-seed" the new 20g tank? (This is always the best way to start a new tank when you can because it gives the new system a 'jump start' in developing the beneficial bacteria colony.)
The fish may have been in poor health and may have been stressed further with the tank (water) changes. I always like to use a drip acclimation* and never add LFS water to my tank.
*for a drip acclimation, you get a bucket or pail for fish that's rinsed well (no soapy mop bucket!). Then use air line tubing and a valve and create a siphon from your main target tank. Adjust the drip so there's a gradual influx of your tank water into the bucket. Let it run until your convinced that a fair amount of your tank water has entered the bucket (uhm...don't let the bucket overflow!). Now, net the fish and transfer to your tank. Discard the water in the bucket. Top off your main tank with fresh water as/if necessary.
This allows your new guest to become slowly acclimated to your water just in case there are water chemistry differences. Typically, fish purchased in your local area are in water very similar to yours...but the water chemistry in different tanks can change for countless reasons so it's always best to slowly acclimate new stock to a new tank.